Enzyme-free Recycling Amplification-based Sensitive Electrochemical Thrombin Aptasensor

In this work, an enzyme-free recycling amplification-based sensitive electrochemical thrombin aptasensor using catalytic hairpin assembly (CHA) and bisferrocene was proposed for electrochemical beacon. Thrombin triggered the CHA reaction that in turn formed a number of double-stranded complexes,which are fixed on the surface of the gold electrode through potential-assisted Au-S deposition, and a large number of labeled bisferrocene are placed close to the gold electrode to generate electrochemical signal. The linear range of the electrochemical sensor was 0.25 pM-2.5 nM for thrombin with detection limit of 0.18 pM. This sensor can be employed to monitor the thrombin in human serum samples.

Automated summary

A sensitive electrochemical thrombin aptasensor was proposed in this work, utilizing CHA and bisferrocene to detect thrombin concentration. The sensor demonstrated a wide linear range and high detection limit, making it suitable for monitoring thrombin in human serum samples.