Exogenous salicylic acid alleviates the accumulation of pesticides and mitigates pesticide-induced oxidative stress in cucumber plants (Cucumis sativus)

Salicylic acid (SA) is an important signal molecule, regulating oxidative stress response in plants. In this study, we evaluated the influences of SA (1 mg L-1, 10 mg L-1 and 50 mg L-1) on the accumulation of clothianidin (CLO), dinotefuran (DFN) and difenoconazole (DFZ) (5 mg L-1) and pesticide-induced (CLO-10 mg L-1, DFN-20 mg L-1, and DFZ-10 mg L-1) oxidative stress in cucumber plants. Exogenous SA at 10 mg L-1 significantly reduced the half-lives of three pesticides in nutrient solution and prevented the accumulation of pesticides in roots and leaves. And the role of SA in reducing residues was related to the major accumulation sites of pesticides. By calculating the root concentration factor (RCF) and translocation factor (TF), we found that SA at 10 mg L-1 reduced the ability of roots to absorb pesticides and enhanced the translocation ability from roots to leaves. Roots exposed to high concentrations of three pesticides could reduce biomass, low chlorophyll content, increase the accumulation of reactive oxygen species (ROS) and proline, promote lipid peroxidation, and alter the activities of a range of antioxidant enzymes, respectively. Exogenous SA at low concentrations (1 mg L-1 and 10 mg L-1) significantly mitigated these negative effects. Hence, application of exogenous SA at 10 mg L-1 could effectively alleviate the accumulation of pesticides and induce stress tolerance in cucumber planting systems.

Automated summary

Exogenous application of salicylic acid at 10 mg L-1 significantly reduced the half-lives of pesticides in nutrient solution, prevented their accumulation in roots and leaves, and enhanced the translocation ability from roots to leaves. Additionally, it mitigated the negative effects of pesticide exposure on plant biomass, chlorophyll content, oxidative stress indicators, and antioxidant enzyme activities.