Ecotoxicity of arsenic contamination toward the soil enchytraeid Enchytraeus crypticus at different biological levels: Laboratory studies

The ecotoxicity of arsenic (As) contamination toward small soil fauna living in soil pore water such as soil enchytraeids has rarely been studied but is important in the assessment of soil pollution. Here, the endpoints of As ecotoxicity to Enchytraeus crypticus were studied at three biological levels, i.e., individual (morphology and body tissue As concentrations), population (survival, reproduction and growth) and cell biochemistry (antioxi-dant enzymes CAT, POD and SOD and peroxidation malondialdehyde MDA). Contact filter paper tests without soil and single species tests with OECD artificial and field soils were conducted. Arsenic contamination resulted in severe morphological pathologies in E. crypticus and the symptoms and degree of damage increased gradually with increasing As concentration and exposure time up to 48 h. The abnormal morphological effects occurred before the impairment of fecundity. The population endpoints responded to the As concentration and the EC50 values increased in the following sequence: reproduction, juvenile body weight, adult body weight, juvenile length and adult length. Changes in biochemistry parameters were induced rapidly and changed with increasing As concentration and exposure time. The activity peak values of enzymes were 3-5 times higher and the activity maximum values of MDA were 1-3 time higher than their controls. The sensitivity of enzyme activities was generally much higher than that of MDA and CAT generally showed the highest enzyme activity. The results indicate that As contamination can be very harmful to soil enchytraeids and the endpoints of the ecotoxicity tests of soil enchytraeids can be used to complement existing soil As assessment systems or may be used alone for the assessment of soil As pollution.

Automated summary

This study investigated the ecotoxicity of arsenic on Enchytraeus crypticus, revealing severe morphological pathologies, impaired reproduction and growth, and altered biochemical parameters in response to arsenic contamination. The results indicate that arsenic contamination can be very harmful to soil enchytraeids, with enzyme activity showing higher sensitivity to arsenic than MDA, and CAT being the enzyme with the highest activity levels. These findings suggest that the ecotoxicity endpoints of soil enchytraeids can be used to evaluate soil arsenic pollution.