Journal
DEVELOPMENT
Volume 143, Issue 9, Pages 1600-1611Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.129098
Keywords
Stomata; RNA polymerase II; Patterning; Differentiation; Arabidopsis
Categories
Funding
- Natural Science Foundation of China (NSFC) [31470372, 31271460, 91017002]
- Ministry of Agriculture of the People's Republic of China [2016ZX08009003-002-009]
- foundation of the Key Laboratory of Cell Activities and Stress Adaptations, Ministry of Education
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Stomata are highly specialized epidermal structures that control transpiration and gas exchange between plants and the environment. Signal networks underlying stomatal development have been previously uncovered but much less is known about how signals involved in stomatal development are transmitted to RNA polymerase II (Pol II or RPB), which plays a central role in the transcription of mRNA coding genes. Here, we identify a partial loss-of-function mutation of the third largest subunit of nuclear DNA-dependent Pol II (NRPB3) that exhibits an increased number of stomatal lineage cells and paired stomata. Phenotypic and genetic analyses indicated that NRPB3 is not only required for correct stomatal patterning, but is also essential for stomatal differentiation. Protein-protein interaction assays showed that NRPB3 directly interacts with two basic helixloop-helix (bHLH) transcription factors, FAMA and INDUCER OF CBF EXPRESSION1 (ICE1), indicating that NRPB3 serves as an acceptor for signals from transcription factors involved in stomatal development. Our findings highlight the surprisingly conserved activating mechanisms mediated by the third largest subunit of Pol II in eukaryotes.
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