Journal
DEVELOPMENT
Volume 143, Issue 7, Pages 1134-1148Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/dev.131060
Keywords
Tctp; tpt1; Neural circuitry assembly; Axon guidance; Retinotectal projection; RNA localisation; Retinal ganglion cell
Categories
Funding
- Fundacao para a Ciencia e Tecnologia [SFRH/BD/33891/2009]
- Sir Edward Youde Memorial Fund
- Croucher Foundation
- Cambridge Commonwealth - European & International Trust
- Gates Cambridge Scholarship
- Wellcome Trust [085314/Z/08/Z]
- Wellcome Trust [085314/Z/08/Z] Funding Source: Wellcome Trust
- Fundação para a Ciência e a Tecnologia [SFRH/BD/33891/2009] Funding Source: FCT
Ask authors/readers for more resources
The transcript encoding translationally controlled tumor protein (Tctp), a molecule associated with aggressive breast cancers, was identified among the most abundant in genome-wide screens of axons, suggesting that Tctp is important in neurons. Here, we tested the role of Tctp in retinal axon development in Xenopus laevis. We report that Tctp deficiency results in stunted and splayed retinotectal projections that fail to innervate the optic tectum at the normal developmental time owing to impaired axon extension. Tctp-deficient axons exhibit defects associated with mitochondrial dysfunction and we show that Tctp interacts in the axonal compartment with myeloid cell leukemia 1 (Mcl1), a pro-survival member of the Bcl2 family. Mcl1 knockdown gives rise to similar axon misprojection phenotypes, and we provide evidence that the anti-apoptotic activity of Tctp is necessary for the normal development of the retinotectal projection. These findings suggest that Tctp supports the development of the retinotectal projection via its regulation of pro-survival signalling and axonal mitochondrial homeostasis, and establish a novel and fundamental role for Tctp in vertebrate neural circuitry assembly.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available