4.4 Article

Restoration of Intestinal Mucosa in Euphorbia kansui-treated Severe Acute Pancreatitis Rats based on HMGB1/MFG-E8 Expression

Journal

CURRENT PHARMACEUTICAL BIOTECHNOLOGY
Volume 22, Issue 12, Pages 1672-1682

Publisher

BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1389201021666201218130440

Keywords

Severe acute pancreatitis; traditional Chinese medicine; intestinal barrier dysfunction; HMGB1; MFG-E8; Euphorbia kansui

Funding

  1. Science and Technology Planning Project of Guangdong Province [2017ZC0180]

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EK treatment effectively reduces the ascites volume, serum amylase and plasma endotoxin levels in SAP rats, as well as significantly decreases abnormal intestinal morphological alterations. EK also inhibits the elevation of inflammatory factors and apoptotic cells in rat ileum tissue, showcasing its potential in suppressing SAP pathogenesis through modulating specific signaling pathways.
Background: The pathogenesis of Severe Acute Pancreatitis (SAP) is mediated substantially by dysfunctions in the intestinal barrier. Euphorbia kansui (EK) is a medicinal plant used widely in traditional Chinese medicine to treat inflammation; however, its efficacy and mechanism of action in SAP treatment are not yet well understood. Objective: To investigate the role of EK in intestinal barrier tissue repair and in the pathogenesis and development of SAP. Methods: The rat SAP model was established by a retrograde injection of sodium taurocholate into the pancreatic bile duct. The SAP model group and the SAP + EK treatment groups were divided into 6 subgroups according to timing: 2, 6, 12, 24, 48, or 72h after inducing SAP. The progression of the SAP rats and of the rats receiving the EK treatment was evaluated using the ascites volume, serum amylase and plasma endotoxin levels, and histological grading of intestinal mucosal damage. In addition, serum inflammatory factor contents were measured using Enzyme-Linked Immunosorbent Assay (ELISA) tests and apoptotic cells in damaged ileum tissue were detected using TUNEL staining. Apoptosis markers and other signaling proteins in intestinal mucosal cells were detected by immunohistochemical assays and then validated by combining these data with quantitative polymerase chain reactions and western blotting. Results: Compared with the results of the SAP model rats, the results of the rats that received EK treatment demonstrated that EK could effectively reduce the ascites volume and serum amylase and plasma endotoxin levels. EK treatment also greatly reduced the abnormal intestinal morphological alterations in the rat SAP model and significantly downregulated the serum contents of Interleukin (IL)-1 beta, IL-6, and tumor necrosis factor-alpha. EK treatment inhibited the elevation of capapse-3, inhibited the decrease of the Bcl-2 protein, and decreased the number of apoptotic cells in rat ileum tissue. Finally, EK treatment abrogated the increase of HMGB1 and the suppression of MFG-E8 protein expression in the SAP + EK rat ileum tissue. Conclusion: EK suppresses SAP pathogenesis by restoring the intestinal barrier function and modulating the HMGB1/MFG-E8 signaling axis.

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