Journal
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
Volume 48, Issue 4, Pages 597-604Publisher
WILEY
DOI: 10.1111/1440-1681.13453
Keywords
gallbladder motility; monochloramine; reactive oxygen species; signal transduction pathways; smooth muscle
Categories
Funding
- Junta de Extremadura, Spain [IB18025, GR18119]
Ask authors/readers for more resources
The study found that the naturally occurring oxidant monochloramine affects guinea pig gallbladder contractions in a concentration-dependent manner, involving various biological pathways such as extracellular Ca2+ influx, tyrosine kinase, PKC, MAPK, and NO pathways. NH2Cl not only impairs the gallbladder response to CCK but also to membrane depolarization and nervous plexus stimulation.
Digestive inflammatory processes induce motility alterations associated with an increase in reactive oxygen species production, including monochloramine (NH2Cl). The aim of the study was to characterize the effects of the naturally occurring oxidant monochloramine in the guinea pig gallbladder. We used standard in vitro contractility technique to record guinea pig gallbladder strips contractions. NH2Cl caused a concentration-dependent contraction which was reduced by inhibition of extracellular Ca2+ influx and tyrosine kinase pathways. The PKC antagonist GF109203X also reduced the response but not after previous tyrosine kinase inhibition, suggesting that PKC is activated by tyrosine kinase activity. The NH2Cl contractile effect was also reduced by inhibitors of mitogen-activated protein kinase (MAPK), nitric oxide synthase, phospholipase A2 and cyclooxygenase. In addition, NH2Cl impaired the responses to CCK, tissue depolarization and electrical field stimulation. In conclusion, we present new evidence that monochloramine impairs not only the gallbladder response to CCK but also to membrane depolarization and nervous plexus stimulation, and that tyrosine kinase, PKC, MAPK and NO pathways are involved in the contractile direct effect of monochloramine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available