Application of adaptive pressure-driven microfluidic chip in thyroid function measurement

The improvement in accuracy of in vitro diagnosis has always been the focus of early screening of thyroid dysfunction. We constructed a microfluidic chip based on a polystyrene polymer substrate. Total triiodothyronine (TT3), total thyroxine (TT4), free triiodothyronine (FT3), free thyroxine (FT4), and thyrotropin (TSH) in human whole blood samples were analysed by fluorescence immunoassay to evaluate thyroid function. The results indicate that the microfluidic chip shows a good linear relationship in the detection of TT3, TT4, FT3, FT4, and TSH standards, and the correlation coefficient (r) is not less than 0.9900. In addition, the chip also has strong anti-interference (RSD% < 5%) and good repeatability (CV < 8%), and its inter-batch differences are small (CV < 15%). The results of practical application in clinical thyroid function measurement indicated its high accuracy (r > 0.9900). It provides a new method for the determination of thyroid function and lays a foundation for subsequent clinical application. (c) 2021 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.

Automated summary

The study developed a microfluidic chip for evaluating thyroid function, showing good accuracy and stability in both experimental and clinical applications. This new method provides a new approach for measuring thyroid function.