Journal
CHEMISTRY-A EUROPEAN JOURNAL
Volume 27, Issue 12, Pages 3986-3990Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.202004760
Keywords
chromophore; fluorescence; fluorescent labeling; fluorogen-activating proteins; green fluorescent protein
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Funding
- Ministry of Science and Higher Education of the Russian Federation [075-15-2020-773]
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By using benzylidene imidazolone core, a range of color-shifted fluorogenic ligands for FAST protein were developed without compromising binding efficiency or utility for live-cell protein labeling. This study emphasizes the potential of benzylidene imidazolone derivatives for rapidly expanding live-cell fluorogenic labeling tools.
Using benzylidene imidazolone core, we created a panel of color-shifted fluorogenic ligands for FAST protein without compromise to the binding efficiency and the utility for live-cell protein labeling. This study highlights the potential of benzylidene imidazolones derivatives for rapid expansion of a pallet of live-cell fluorogenic labeling tools.
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