Journal
CHEMBIOCHEM
Volume 22, Issue 9, Pages 1676-1685Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202000845
Keywords
fluorescent probes; peptides; pyrene; reactive oxygen species; time-resolved spectroscopy
Funding
- Occitanie region
- Julius-Maximilians-Universitat Wurzburg
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Real-time quantification of reactive nitrogen and oxygen species (ROS) in cells is crucial for cellular functions, as excessive formation can lead to cell dysfunction and death. Researchers have synthesized fluorescent probes that can target mitochondria to detect and quantify the variation of ROS in real time.
Real-time quantification of reactive nitrogen and oxygen species (ROS) in cells is of paramount importance as they are essential for cellular functions. Their excessive formation contributes to the dysfunction of cells and organisms, ultimately leading to cell death. As ROS are mostly produced in the mitochondria, we have synthesized a fluorescent probe able to reach this organelle to detect and quantify, in real time, the variation of ROS by time-resolved microfluorimetry. The new probes are based on the long fluorescence lifetime of pyrene butyric acid (PBA). Two PBA isomers, attached at their 1- or 2-positions to a peptide vector to target mitochondria, were compared and were shown to allow the measurement of free radical species and oxygen, but not non-radical species such as H2O2.
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