ER-mitochondria contact sites in neurodegeneration: genetic screening approaches to investigate novel disease mechanisms

Mitochondria-ER contact sites (MERCS) are known to underpin many important cellular homoeostatic functions, including mitochondrial quality control, lipid metabolism, calcium homoeostasis, the unfolded protein response and ER stress. These functions are known to be dysregulated in neurodegenerative diseases, including Parkinson's disease (PD), Alzheimer's disease (AD) and amyloid lateral sclerosis (ALS), and the number of disease-related proteins and genes being associated with MERCS is increasing. However, many details regarding MERCS and their role in neurodegenerative diseases remain unknown. In this review, we aim to summarise the current knowledge regarding the structure and function of MERCS, and to update the field on current research in PD, AD and ALS. Furthermore, we will evaluate high-throughput screening techniques, including RNAi vs CRISPR/Cas9, pooled vs arrayed formats and how these could be combined with current techniques to visualise MERCS. We will consider the advantages and disadvantages of each technique and how it can be utilised to uncover novel protein pathways involved in MERCS dysfunction in neurodegenerative diseases.

Automated summary

Mitochondria-ER contact sites (MERCS) play crucial roles in cellular homeostasis and are linked to neurodegenerative diseases, yet many details about their involvement in these diseases remain unknown. This review aims to summarize the current knowledge on MERCS structure and function, as well as evaluate high-throughput screening techniques for uncovering novel protein pathways related to MERCS dysfunction in neurodegenerative diseases.