Journal
CELL
Volume 183, Issue 7, Pages 1801-+Publisher
CELL PRESS
DOI: 10.1016/j.cell.2020.11.010
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Funding
- Novartis Research Foundation
- Swiss National Science Foundation [31003A_182314]
- SNF-NCCR RNA Disease
- EMBO fellowship [ALTF 1203-2018]
- European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Grant [843212]
- Swiss National Science Foundation (SNF) [31003A_182314] Funding Source: Swiss National Science Foundation (SNF)
- Marie Curie Actions (MSCA) [843212] Funding Source: Marie Curie Actions (MSCA)
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Cellular stress leads to reprogramming of mRNA translation and formation of stress granules (SGs), membraneless organelles consisting of mRNA and RNA-binding proteins. Although the function of SGs remains largely unknown, it is widely assumed they contain exclusively non-translating mRNA. Here, we re-examine this hypothesis using single-molecule imaging of mRNA translation in living cells. Although we observe non-translating mRNAs are preferentially recruited to SGs, we find unequivocal evidence that mRNAs localized to SGs can undergo translation. Our data indicate that SG-associated translation is not rare, and the entire translation cycle (initiation, elongation, and termination) can occur on SG-localized transcripts. Furthermore, translating mRNAs can be observed transitioning between the cytosol and SGs without changing their translational status. Together, these results demonstrate that mRNA localization to SGs is compatible with translation and argue against a direct role for SGs in inhibition of protein synthesis.
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