Effect of integrated treatment on improving the enzymatic digestibility of poplar and the structural features of isolated hemicelluloses

Herein, a two-step hydrothermal pretreatment combined with alkali extraction method was applied to deconstruct the poplar cell walls for enzymatic hydrolysis. Results revealed that 88.1 % of hemicelluloses and 77.6 % of lignin were removed during the integrated treatment performed at 180 degrees C and a maximum enzymatic hydrolysis efficiency of 96.1 % was achieved. Confocal Raman microscopy suggested that the removal of hemicelluloses from cell walls was inhomogeneous, and most hemicelluloses were released from the secondary wall. In addition, 35.2-56.8 % of hemicelluloses were isolated from the integrated treatment. Detailed structural analysis revealed that the water-soluble hemicelluloses possessed more branched structure than the alkali-soluble hemicelluloses and the hemicelluloses isolated from the poplar were mainly composed of a linear backbone of (1 -> 4)-beta-D-Xylp with 4-O-Me-alpha-d-GlcpA attached as side chains. This work provides an efficient pathway to transform poplar into fermentable sugars and hemicelluloses with considerable yield.

Automated summary

In this study, a two-step hydrothermal pretreatment combined with alkali extraction method was used to deconstruct poplar cell walls for enzymatic hydrolysis, resulting in the removal of a significant amount of hemicelluloses and lignin. Confocal Raman microscopy revealed that hemicelluloses were unevenly removed from cell walls, mainly originating from the secondary wall. Water-soluble hemicelluloses were found to have a more branched structure compared to alkali-soluble hemicelluloses, with a linear backbone of (1 -> 4)-beta-D-Xylp and 4-O-Me-alpha-d-GlcpA side chains identified in hemicelluloses isolated from poplar. This work provides an efficient pathway to convert poplar into fermentable sugars and hemicelluloses with significant yield.