Journal
CARBOHYDRATE POLYMERS
Volume 250, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.117014
Keywords
Hyaluronic acid; Oligosaccharides; Polysaccharide derivative; Mass spectrometric analysis; Liquid chromatography analysis; Glycosaminoglycans
Categories
Funding
- Palacky University in Olomouc [IGA_PrF_2020_030]
- Ministry of Education, Youth, and Sports of the Czech Republic (ERDF project Nanotechnologies for future) [CZ.02.1.01/0.0/0.0/16_019/0000754]
Ask authors/readers for more resources
The aim of this paper is to review chromatographic and mass-spectrometric methods and underline the best analytical approaches for successful analysis of various hyaluronic acid species in different types of samples. Hyaluronan-degrading enzymes and chemical depolymerization produce dior oligosaccharides suitable for hyaluronan quantification or structural characterization of hyaluronan derivatives. Efficient purification and pre-column derivatization of hyaluronan disaccharides by reductive amination allow subnanogram quantification in biological samples. The chromatographic separation is capable to distinguish all glycosaminoglycans disaccharides and to resolve hyaluronan fragments with 2-40 monomers. Using electrospray ionization or matrix assisted laser desorption ionization, hyaluronan fragments up to 8 kDa or 41 kDa, respectively, can be observed. One- or two-dimensional chromatographic separation with higly sensitive mass-spectrometric detection is an indispensable tool for revealing substituent position, extent of modification and substitution patterns of chemically modified hyaluronan derivatives. It is essential for studying structure-biological function relationships of hyaluronan and its derivatives.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available