Pectin co-functionalized dual layered solid lipid nanoparticle made by soluble curcumin for the targeted potential treatment of colorectal cancer

The investigation is to increase the cytotoxicity of soluble curcumin (SC) by loading it onto pectin and skimmed milk powder (SMP) dual layered solid lipid nanoparticles (DL-SLN). The DL-SLN exhibited significantly higher encapsulation efficiency (83.94 +/- 6.16), better stability (90 days), and sustained the drug release in different gastro intestional (GI) environments upto 72 h. Molecular docking revealed that the Vander Waals (57420.669 Kcal-mol(-1)) and electrostatic (-197.533) bonds were involved in the DL-SLN complex formation. The in vivo toxicity of DL-SLN was performed by the zebrafish model, the cell cycle arrest at G2/M phase (64.34 %) by flow cytometry, and western blot investigation was recognized molecular level cell death using SW480 cells. Pharmacokinetic (PK) evaluation (Cmax-5.78 +/- 3.26 mu g/mL; Tmax-24 h) and organ distribution studies confirmed that the co-functionalized pectin based SLN could efficiently improve the oral bioavailability (up to 72 h) of curcumin (CMN) on colon-targeted release.

Automated summary

The study aimed to increase the cytotoxicity of soluble curcumin by loading it onto pectin and skimmed milk powder dual layered solid lipid nanoparticles. The dual layered solid lipid nanoparticles showed improved stability and sustained drug release in different gastrointestinal environments. In vivo toxicity was evaluated using a zebrafish model, cell cycle arrest at G2/M phase was observed, and pharmacokinetic evaluation confirmed improved oral bioavailability of curcumin on colon-targeted release.