Characterization of an oxidized alginate-gelatin hydrogel incorporating a COS-salicylic acid conjugate for wound healing

In the present study, chitooligosaccharide (COS) and salicylic acid (SA) conjugates were synthesized using H2O2-induced grafting polymerization and oxidized alginate (OA) and gelatin hydrogels were fabricated using Schiff's base reaction. To confirm the synthesis and improved activity of the COS-SA conjugates, polyphenol assays, ultraviolet absorbance, NMR, and antioxidant activity tests were all conducted. The results showed that the COS-SA conjugates were completely synthesized and improved antioxidant activity. OA was synthesized using a periodate method and a hydrogel was obtained at a mixed ratio of 3:7 via crosslinking between the aldehyde group of the OA and the amine groups of gelatin with COS-SA conjugates. In a cytotoxicity and wound healing model, the hydrogels with COS-SA conjugates did not exhibit cytotoxicity and exhibited wound healing activity in a mouse model. These results provide evidence that OA and gelatin hydrogels with a COS-SA conjugate can be successfully used for wound healing applications.

Automated summary

In this study, chitooligosaccharide (COS) and salicylic acid (SA) conjugates were synthesized and oxidized alginate (OA) and gelatin hydrogels were fabricated. Tests confirmed successful synthesis of COS-SA conjugates with improved antioxidant activity. The hydrogels showed non-cytotoxicity and wound healing activity, indicating their potential for wound healing applications.