Journal
CARBOHYDRATE POLYMERS
Volume 251, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2020.117056
Keywords
Starch; Maltogenic alpha-amylase; Transglucosidase; Solid-state modification
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Funding
- Archer Daniels Midland (ADM) Company, Decatur IL
- China Scholarship Council (CSC)
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The study demonstrates the potential of sequential addition of starch active enzymes, MA and TGA, to obtain granular starch with improved functionality.
We describe a method for permitting efficient modification by transglucosidase (TGA), from glycoside hydmlase family 31 (GH31), sequentially after the pre-treatment by maltogenic alpha-amylases (MA) from GH13. TGA treatment without MA pre-treatment had negligible effects on native starch, while TGA treatment with MA pretreatment resulted in porous granules and increased permeability to enzymes. MA -> TGA treatments lead to decreased molecular size of amylopectin molecules, increased alpha-1,6 branching, and increased amounts of amylopectin chains with the degree of polymerization (DP)<10 and decreased amounts of DP 10-28 after debranching. Wide-angle X-ray scattering (WAXS) data showed a general decrease in crystallinity except for a long term (20 h) TGA post-treatment which increased the relative crystallinity back to normal. MA -> TGA treatment significantly lowered the starch retrogradation of starch and retarded the increase of storage- and loss moduli during storage. This work demonstrates the potential of sequential addition of starch active enzymes to obtain granular starch with improved functionality.
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