The impact of epitranscriptomic marks on post-transcriptional regulation in plants

Ribonucleotides within the various RNA molecules in eukaryotes are marked with more than 160 distinct covalent chemical modifications. These modifications include those that occur internally in messenger RNA (mRNA) molecules such as N-6-methyladenosine (m(6)A) and 5-methylcytosine (m(5)C), as well as those that occur at the ends of the modified RNAs like the non-canonical 5'' end nicotinamide adenine dinucleotide (NAD(+)) cap modification of specific mRNAs. Recent findings have revealed that covalent RNA modifications can impact the secondary structure, translatability, functionality, stability and degradation of the RNA molecules in which they are included. Many of these covalent RNA additions have also been found to be dynamically added and removed through writer and eraser complexes, respectively, providing a new layer of epitranscriptome-mediated post-transcriptional regulation that regulates RNA quality and quantity in eukaryotic transcriptomes. Thus, it is not surprising that the regulation of RNA fate mediated by these epitranscriptomic marks has been demonstrated to have widespread effects on plant development and the responses of these organisms to abiotic and biotic stresses. In this review, we highlight recent progress focused on the study of the dynamic nature of these epitranscriptome marks and their roles in post-transcriptional regulation during plant development and response to environmental cues, with an emphasis on the mRNA modifications of non-canonical 5' end NAD(+) capping, m(6)A and several other internal RNA modifications.

Automated summary

Eukaryotic RNA molecules are marked with over 160 distinct covalent chemical modifications, impacting their structure, translatability, functionality, stability, and degradation. These modifications are dynamically added and removed through writer and eraser complexes, providing a new layer of epitranscriptome-mediated post-transcriptional regulation in eukaryotic transcriptomes. The regulation of RNA fate mediated by these epitranscriptomic marks has widespread effects on plant development and their responses to environmental cues.