Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 118, Issue 3, Pages 1366-1380Publisher
WILEY
DOI: 10.1002/bit.27658
Keywords
Clostridium pasteurianum; formate assimilation; glycine synthase
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Funding
- Projekt DEAL
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The study successfully introduced the glycine synthase system genes from Gottschalkia acidurici into Clostridium pasteurianum, resulting in changes in product metabolism. Activation of glycine synthase led to the secretion of 2-oxobutyrate as a metabolic intermediate of Clostridium pasteurianum for the first time. These findings contribute to a better understanding of the metabolic pathways of Clostridium pasteurianum.
Autotrophic or mixotrophic use of one-carbon (C1) compounds is gaining importance for sustainable bioproduction. In an effort to integrate the reductive glycine pathway (rGP) as a highly promising pathway for the assimilation of CO2 and formate, genes coding for glycine synthase system from Gottschalkia acidurici were successfully introduced into Clostridium pasteurianum, a non-model host microorganism with industrial interests. The mutant harboring glycine synthase exhibited assimilation of exogenous formate and reduced CO2 formation. Further metabolic data clearly showed large impacts of expression of glycine synthase on the product metabolism of C. pasteurianum. In particular, 2-oxobutyrate (2-OB) was observed for the first time as a metabolic intermediate of C. pasteurianum and its secretion was solely triggered by the expression of glycine synthase. The perturbation of C1 metabolism is discussed regarding its interactions with pathways of the central metabolism, acidogenesis, solventogenesis, and amino acid metabolism. The secretion of 2-OB is considered as a consequence of metabolic and redox instabilities due to the activity of glycine synthase and may represent a common metabolic response of Clostridia in enhanced use of C1 compounds.
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