4.8 Article

Copper peroxide/ZIF-8 self-producing H2O2 triggered cascade reaction for amperometric immunoassay of carbohydrate antigen 19-9

Journal

BIOSENSORS & BIOELECTRONICS
Volume 169, Issue -, Pages -

Publisher

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112644

Keywords

Amperometric immunosensor; Carbohydrate antigen 19-9; Copper peroxide/ZIF-8; Self-producing H2O2; Fenton-type reaction; Cascade reaction

Funding

  1. National Natural Science Foundation of China [21673143]
  2. Natural Science Foundation of Beijing Municipality [2172016]
  3. Capacity Building for Sci-Tech Innovation-Fundamental Scientific Research Funds [20530290087, 20530290055]

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Generally, H2O2 is frequently adopted to improve analysis capabilities of various detection systems. However, the addition of H2O2 with relatively higher concentration can lower the bioactivity of antibodies or antigens and the sensing interface stability in most peroxidase and peroxidase-like immunosensors. In order to solve these issues, we designed a novel copper peroxide/ZIF-8 immunoprobe that can self-produce H2O2 to trigger a cascade reaction for the sensitive detection of carbohydrate antigen 19-9. Specifically, CP/ZIF-8 plays a key role as a signal switch in the immunosensor. In the presence of HCl, the structures of ZIF-8 and copper peroxide can be broken, producing Cu2+ and H2O2 and a subsequent Fenton-type reaction that generates center dot OH. The resulting center dot OH can induce the decomposition of 3-aminobenzeneboronic acid/poly (vinyl alcohol) (PVA) film on the electrode. Although the immunosensor initially showed little current signal due to the poor conductivity pf ZIF-8 and PVA, the current signal was significantly amplified by a HCl-triggered cascade reaction. Under optimal conditions, the immunosensor displayed a wide linear range from 0.0001 to 100 U mL(-)(1) with an ultralow limit of detection of 53.5 mu U mL(-1) (S/N = 3) for carbohydrate antigen 19-9. Considering these advantages, namely self-producing H2O2 and easy operation, this strategy paves a new way to design other novel sensors.

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