Promoting anaerobic digestion by algae-based hydrochars in a continuous reactor

The microalgae and macroalgae-based hydrochars produced by hydrothermal carbonization were mainly used as biofuels, however, their application in anaerobic digestion (AD) was little known. This study investigated the effects of microalgae Chlorella-based hydrochar (HC-C) and macroalgae Laminaria-based hydrochar (HC-L) on a continuous AD reactor under different organic loading rates (OLR). The AD process stability of hydrochars supplemented reactors were performed well under the increase of OLR from 2.6 to 6.5 g COD/L/d, and HC-C and HC-L addition could significantly enhance the daily methane yield by 36.0% and 31.4%, respectively. Interestingly, the possible mechanisms of HC-C and HC-L on the enhanced AD were similar, namely increasing sludge granulation, promoting the Methanothrix relative abundance and key enzyme activities, and further facilitating potential direct interspecies electron transfer between methanogens and organic-degrading bacteria. This study provided an implication on the potential application of algae-based hydrochars in wastewater treatment and energy recovery.