Sodium hydroxide catalytic ethanol pretreatment and surfactant on the enzymatic saccharification of sugarcane bagasse

The effect of NaOH catalytic ethanol pretreatment under various temperatures (130-180 degrees C) and time (15-90 min) on the chemical composition and enzymatic saccharification of sugarcane bagasse was investigated in this study. The results showed that NaOH catalytic ethanol pretreatment assisted delignification and the reservation of cellulose and hemicellulose. When sugarcane bagasse was pretreated at 180 degrees C for 30 min, a substantial glucose yield of 91.6% was obtained after hydrolysis for 72 h, representing 94.6% of glucose in pretreated residue. This yield was promoted with respect to the compositional change and surface alteration of pretreated substrate. With the supplement of Tween 80, the enzyme usage would be saved by 50% and the enzymolysis time could be shortened to 24 h while obtaining comparable glucose yield. This study provided an economical feasible and gradual process for the generation of glucose, which was followed by fermentation and conversion to platform chemicals.

Automated summary

The NaOH catalytic ethanol pretreatment of sugarcane bagasse improved glucose yield by assisting delignification and preserving cellulose and hemicellulose. Higher temperature and longer time resulted in higher glucose yield, while the addition of Tween 80 significantly reduced enzyme usage and enzymolysis time while maintaining comparable glucose yield. This study provided an economically feasible method for generating glucose followed by fermentation and conversion to platform chemicals.