Journal
BIORESOURCE TECHNOLOGY
Volume 319, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.124159
Keywords
Cellulase; Immobilization; In situ saccharification; Lignocellulosic biomass; Surface charge
Funding
- National Natural Science Foundation of China [21676173, :31770903]
- Agricultural Infrastructure Project of Suzhou Science and Technology Development Plan [SNG2018046]
- Graduate Research and Innovation Projects of Jiangsu Province [KYCX20_2776]
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Modifying the surface charge on biomass components to alter cellulase affinity is an urgent requisite due to the intrinsic recalcitrance of lignocellulosic biomass. Charge plays a crucial role in affecting the interaction between immobilized cellulase and biomass in situ saccharification, and further investigation on the impact of charge on cellulase immobilization is essential. Strategies involving charge tunable cellulase and immobilized cellulase show promising pathways to improve in situ biomass saccharification.
The intrinsic recalcitrance of lignocellulosic biomass makes it resistant to enzymatic hydrolysis. The electron-rich surface of the lignin and cellulose-alike structure of hemicellulose competitively absorb the cellulase. Thus, modifying the surface charge on biomass components to alter cellulase affinity is an urgent requisite. Developing charge tunable cellulase will alter substrate affinity. Also, charge-based immobilization generates controllable substrate affinity. Within immobilized cellulase involved in situ biomass saccharification, charge effects made a crucial contribution. In addition to affecting the interaction between immobilized cellulase and biomass, charge exerts an impact on cellulase to immobilize the materials, further investigation is essential. This study aims to review the charge effects on the cellulase affinity in biomass saccharification, strategies of charge tunable cellulase, and immobilized cellulase, thereby explaining the role of electrostatic interaction. In terms of electrostatic behavior, the pathways and plans to improve in situ biomass saccharification seem to be promising.
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