Thermal pretreatment enhances the degradation and humification of lignocellulose by stimulating thermophilic bacteria during dairy manure composting

This study investigated the effect of thermal pretreatment (TP) on the lignocellulose degradation and humification during dairy manure composting and the underlying microbial mechanism. The results showed that TP accelerated temperature rise and elevated composting temperature by increasing 26% initial content of simple organics. The degradation of cellulose, hemicellulose and lignin was 78, 10 and 109% higher in thermal pretreatment composting (TPC) than traditional composting (TC), respectively. Moreover, TP significantly improved the humification degree of composts, as indicated by 14 and 38% higher humus content and humification indexes in TPC, respectively. 16S rRNA sequencing showed that TP increased the relative abundance of thermophilic bacteria in TPC, of which Thermobifida, Planifilum, Truepera and Thermomonospora were potentially involved in lignocellulose biodegradation and humification. Canonical correspondence analysis revealed that TP changed the main factor determining the bacterial community evolution from dissolved organic carbon (DOC) in TC to temperature in TPC.

Automated summary

The study demonstrated that thermal pretreatment accelerated temperature rise and enhanced degradation of cellulose, hemicellulose, and lignin during dairy manure composting. Additionally, thermal pretreatment significantly improved humification degree and increased the abundance of thermophilic bacteria involved in lignocellulose degradation and humification. Canonical correspondence analysis indicated that thermal pretreatment shifted the main factor affecting bacterial community evolution from dissolved organic carbon in traditional composting to temperature in thermal pretreatment composting.