Journal
BIOMICROFLUIDICS
Volume 15, Issue 1, Pages -Publisher
AIP Publishing
DOI: 10.1063/5.0039146
Keywords
-
Categories
Funding
- Canon U.S. Life Sciences Inc.
- National Institutes of Health (NIH) [R01GM130923]
- National Science Foundation (NSF) [ECCS1609074]
Ask authors/readers for more resources
This study demonstrates the seamless integration of multiple reagents into thermoplastic 2D microwell arrays using a scalable pin spotting technique, allowing for high-resolution deposition of concentrated reagents. The performance of this integration method was successfully characterized, showing potential for future applications in disposable diagnostics.
The seamless integration of reagents into microfluidic devices can serve to significantly reduce assay complexity and cost for disposable diagnostics. In this work, the integration of multiplexed reagents into thermoplastic 2D microwell arrays is demonstrated using a scalable pin spotting technique. Using a simple and low-cost narrow-bore capillary spotting pin, high resolution deposition of concentrated reagents within the arrays of enclosed nanoliter-scale wells is achieved. The pin spotting method is further employed to encapsulate the deposited reagents with a chemically modified wax layer that serves to prevent disruption of the dried assay components during sample introduction through a shared microchannel, while also enabling temperature-controlled release after sample filling is complete. This approach supports the arbitrary patterning and release of different reagents within individual wells without crosstalk for multiplexed analyses. The performance of the in-well spotting technique is characterized using on-chip rolling circle amplification to evaluate its potential for nucleic acid-based diagnostics. Published under license by AIP Publishing.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available