Biomedical Applications of Mycosynthesized Selenium Nanoparticles Using Penicillium expansum ATTC 36200

In this study, green and eco-friendly biosynthesis of selenium nanoparticles (Se-NPs) were performed using Penicillium expansum ATTC 36200 for multiple biomedical applications. Mycosynthesized Se-NPs were completely characterized using UV, FT-IR, XRD, SEM, and TEM techniques. Se-NPs biosynthesized by P. expansum was characterized as a spherical shape with average size 4 to 12.7 nm. Moreover, Se-NPs were evaluated for multiple biomedical applications as antimicrobial, antioxidant, and anticancer activities and hemocompatibility. Results illustrated that Se-NPs have potential antimicrobial activity against Gram-positive (Bacillus subtilis ATCC6051 and Staphylococcus aureus ATCC23235), Gram-negative bacteria (Escherichia coli ATCC8739and Pseudomonas aeruginosa ATCC9027), fungi (Candida albicans ATCC90028, Aspergillus niger RCMB 02724 and Aspergillus fumigatus RCMB 02568), and antioxidant activity. Additionally, Se-NPs exhibited anticancer activity against PC3 cell line; IC50 was 99.25 mu g/mL. Meanwhile, they showed non-hemolytic activity on human RBCs at concentration up to 250 mu g/mL. In conclusion, biosynthetic Se-NPs by P. expansum are promising for many safe-use biomedical applications.

Automated summary

The biosynthesis of selenium nanoparticles (Se-NPs) using Penicillium expansum ATTC 36200 for biomedical applications showed promising antimicrobial, antioxidant, anticancer activities, and hemocompatibility. The Se-NPs exhibited potential antimicrobial activity against various bacteria and fungi, as well as antioxidant properties. Additionally, they displayed anticancer activity against PC3 cell line and non-hemolytic activity on human RBCs, suggesting their potential for safe biomedical applications.