Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 537, Issue -, Pages 93-99Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2020.12.079
Keywords
LSR; Epithelial ovarian cancer; AMPK; LKB1; Cell survival; Energy stress
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Funding
- Japan Agency for Medical Research and Development (AMED) [JP17im0210606, JP20im0210111]
- Japanese Ministry of Education, Science, Sports, and Culture [16K14637]
- Grants-in-Aid for Scientific Research [16K14637] Funding Source: KAKEN
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The study found that LSR promotes EOC cell survival and tumor growth through the LKB1-AMPK pathway, regulating the activity of AMPK and ACC under glucose deprivation conditions.
Lipolysis-stimulated lipoprotein receptor (LSR), also known as a component of tricellular tight junctions, is highly expressing in epithelial ovarian cancer (EOC). However, the biological role of LSR in EOC cells remains unclear. In this study, we evaluated liver kinase B1 (LKB1) mediated AMP-activated protein kinase (AMPK) activity and investigated the effect of LSR on EOC cell survival under energy stress. LSR increased the levels of phospho-AMPK alpha at Thr172 and phospho-acetyl-CoA carboxylase (ACC) at Ser79 via LKB1-AMPK pathway in glucose deprivation in vitro. The increase of P-AMPK alpha (Thr172) and P-ACC (Ser79) was also detected in tumor microenvironment in vivo. Meanwhile, LSR promoted LKB1 localization at the cell membrane of EOC cells. By cell survival analysis, LSR attenuated glucose deprivation induced cell death in EOC cells in vitro. Our results suggest that LSR promotes EOC cell survival and tumor growth through the LKB1-AMPK pathway. (C) 2020 Elsevier Inc. All rights reserved.
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