Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 534, Issue -, Pages 387-394Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2020.11.072
Keywords
Glycation; Amyloidogenesis; Carboxymethyl lysine; Secondary structure; Oxidative stress; EPR; Aggregation
Categories
Funding
- SASTRA University
- Department of Biotechnology, Government of India [BT/PR9167/BRB/10/1268/2013]
- DST-FIST [SR/FST/ETI-331/2013, R&M/0021/SCBT-007/2012-13]
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The study revealed the impact of glycation by Pyruvic acid on the conformational changes in Hemoglobin, leading to reduction of heme center and formation of advanced glycation end products. The role of pyruvic acid in increasing oxidative stress and diabetic complications was also highlighted.
The effect of glycation by Pyruvic acid (PA) on the early and advanced conformational changes in Hemoglobin (Hb) was studied. Multi Spectroscopic measurement revealed that Hb undergoes structural conformational changes and unbound heme upon incubation with PA. These covalent modifications were followed by the reduction of heme centre and these reduction processes initiates its peroxidase-like activity. An extended PA glycation resulted in the appearance of advanced glycation end products fluorescence, with notable changes in compositions of secondary structure. The amyloidogenic state was confirmed by SEM, fluorescence microscope observation. This study reveals an insight to the role of pyruvic acid which increases the oxidative stress due to the heme reduction and diabetic complication. (C) 2020 Elsevier Inc. All rights reserved.
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