Journal
BASIC RESEARCH IN CARDIOLOGY
Volume 116, Issue 1, Pages -Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00395-020-00839-3
Keywords
Animal models; CRISPR/Cas; Genome editing; Animal models
Categories
Funding
- Projekt DEAL
- DGK
- DZHK
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Gene editing has evolved from a scientific concept to a widely used technology in laboratories, with revolutionary implications for basic science and clinical treatment. Researchers are striving to advance the field by developing new techniques and combining cellular and animal applications to address challenges in gene editing.
For a long time, gene editing had been a scientific concept, which was limited to a few applications. With recent developments, following the discovery of TALEN zinc-finger endonucleases and in particular the CRISPR/Cas system, gene editing has become a technique applicable in most laboratories. The current gain- and loss-of function models in basic science are revolutionary as they allow unbiased screens of unprecedented depth and complexity and rapid development of transgenic animals. Modifications of CRISPR/Cas have been developed to precisely interrogate epigenetic regulation or to visualize DNA complexes. Moreover, gene editing as a clinical treatment option is rapidly developing with first trials on the way. This article reviews the most recent progress in the field, covering expert opinions gathered during joint conferences on genome editing of the German Cardiac Society (DGK) and the German Center for Cardiovascular Research (DZHK). Particularly focusing on the translational aspect and the combination of cellular and animal applications, the authors aim to provide direction for the development of the field and the most frequent applications with their problems.
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