Journal
AQUATIC TOXICOLOGY
Volume 230, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aquatox.2020.105679
Keywords
Triclosan; Mosquitofish; Nrf2; Sirtuin; RedoximiRs
Categories
Funding
- National Natural Science Foundation of China [41576110, 31770554]
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This study investigated the transcriptional responses of Nrf2 and its downstream genes, sirtuins, and RedoximiRs in the livers of mosquitofish exposed to environmentally relevant concentrations of TCS. Results showed that TCS induced oxidative stress in mosquitofish and modulated the antioxidant system through the Nrf2/ARE signaling pathway. The study suggests potential profound effects of TCS on aquatic ecological safety via the RedoximiRs/Sirtuin/Nrf2/ARE signaling pathway.
Triclosan (TCS) has been widely used in daily life for its broad-spectrum antibacterial property and subsequently detected frequently in aquatic waterborne. Environmental relevant concentrations of TCS in water (ng-mu g/L) may pose potential unexpected impact on non-target aquatic organisms. In the present work, we investigated the transcriptional responses of Nrf2 as well as its downstream genes, sirtuins and redox-sensitive microRNAs (RedoximiRs) in livers of the small freshwater fish mosquitofish (Gambusia affinis) which were exposed to environmental relevant concentrations of TCS (0.05 mu g/L, 0.5 mu g/L and 5 mu g/L for 24 h and 168 h). Results showed there were similar up-regulations in Nrf2 and its target genes (e. g. NQO1, CAT and SOD) at transcriptional, enzymatic and protein levels, reflecting oxidative stress of TCS to mosquitofish. Meanwhile, upregulations of Sirt1, Sirt2 and down-regulations of miR-34b, miR-200b-5p and miR-21 could modulate antioxidant system via the Nrf2/ARE signaling pathway by the post-transcriptional regulations. Some oxidative stress-related biomarkers displayed in concentration-dependent manners (e. g. NQO1 mRNA, CAT mRNA) and/or time-dependent manners (e. g. GSH contents). This study indicated that the RedoximiRs/Sirtuin/Nrf2/ARE signaling pathway played a crucial role in mosquitofish exposed to TCS, and there might be potentially profound effects for TCS on the aquatic ecological safety.
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