Journal
AQUACULTURE
Volume 531, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.aquaculture.2020.735991
Keywords
Giant clam; Species identification; Multiplex species-specific PCR; COI
Categories
Funding
- National Science Foundation of China [31702340, 31872566]
- Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0402]
- National Key R&D Program of China [2018YFC1406505, 2018YFD0901400]
- Strategic Pilot Project of the Chinese Academy of Sciences [XDA13020202, XDA13020403]
- Science and Technology Program of Guangzhou, China [201804020073]
- Open Foundation of State Key Laboratory of Loess and Quaternary Geology [SKLLQG1813]
- Innovation Academy of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences [ISEE2018PY01, ISEE2018ZD02, ISEE2018PY03]
- Chinese Academy of Science and Technology Service Network Planning [KFJ-STS-QYZD-158]
- China Agricultural Shellfish Industry Technology System Project [CARS-49]
- Science and Technology Planning Project of Guangdong Province, China [2017B030314052]
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This study demonstrates the utility of a multiplex species-specific PCR for identifying five giant clam species in the South China Sea based on mitochondrial COI gene markers. The approach allows for rapid, reliable, and cost-effective identification, improving the efficiency and quality of giant clam stock management, protection, and forensic identification by reducing the risk of misidentification.
In an effort to develop molecular tools for giant clam species identification, this study reveals the usefulness of a multiplex species-specific PCR for the reliable, rapid and low-cost identification of five giant clam species found in the South China Sea: Hippopus hippopus, Tridacna maxima, Tridacna derasa, Tridacna squamosa and Tridacna noae. We used the mitochondrial COI gene for marker development. Five reverse primers and a common forward primer were designed to generate five different size species-specific PCR fragments. These six primers were combined in a multiplex PCR to enable identification of five giant clam species in a single reaction. This molecular approach provides a simple and rapid identification of the giant clam species from the South China Sea, thus increasing the efficiency and quality of giant clam stock management, protecton and forensic identification by reducing the risk of wrong species identification.
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