Progress of cationic gene delivery reagents for non-viral vector

Gene delivery systems play a vital role in gene therapy and recombinant protein production. The advantages of using gene delivery reagents for non-viral vector include the capacity to accommodate a large packaging load and their low or absent immunogenicity. Furthermore, they are easy to produce at a large scale and preserve. Gene delivery reagents for non-viral vector are commonly used for transfecting a variety of cells and tissues. It is mainly composed of liposomes and non-liposome cationic polymers. According to the different head structures used, the non-viral cationic transfection reagents include a quaternary ammonium salt, amine, amino acid or polypeptide, guanidine salt, and a heterocyclic ring. This article summarizes these approaches and developments of types and components of transfection reagents and optimization of gene delivery. The optimization of mammalian cell transient recombinant protein expression system and cationic reagents for clinical or clinical trials are also discussed.

Automated summary

Gene delivery systems are crucial for gene therapy and recombinant protein production, with non-viral vector gene delivery reagents offering advantages such as large packaging load capacity and low immunogenicity. Liposomes and non-liposome cationic polymers are commonly used as gene delivery reagents, with different head structures including quaternary ammonium salt, amine, amino acid, polypeptide, guanidine salt, and a heterocyclic ring.