Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 105, Issue 1, Pages 1-12Publisher
SPRINGER
DOI: 10.1007/s00253-020-10971-8
Keywords
Small regulatory RNA; Synthetic sRNA; Gene knockdown; Synthetic sRNA applications; Synthetic sRNA improvements; Metabolic engineering
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Funding
- National Natural Science Foundation of China [31971197, 31500086]
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Key points center dot Synthetic sRNAs can be rationally designed based on modular structures of natural sRNAs. center dot Synthetic sRNAs were widely used for metabolic engineering in various microorganisms. center dot Several technological improvements made the synthetic sRNA strategy more applicable.
Small regulatory RNAs (sRNAs) finely control gene expression in prokaryotes and synthetic sRNA has become a useful high-throughput approach to tackle current challenges in metabolic engineering because of its many advantages compared to conventional gene knockouts. In this review, we first focus on the modular structures of sRNAs and rational design strategies of synthetic sRNAs on the basis of their modular structures. The wide applications of synthetic sRNAs in bacterial metabolic engineering, with or without the aid of heterogeneously expressed Hfq protein, were also covered. In addition, we give attention to the improvements in implementing synthetic sRNAs, which make the synthetic sRNA strategy universally applicable in metabolic engineering and synthetic biology.Key points center dot Synthetic sRNAs can be rationally designed based on modular structures of natural sRNAs.center dot Synthetic sRNAs were widely used for metabolic engineering in various microorganisms.center dot Several technological improvements made the synthetic sRNA strategy more applicable.
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