Pseudocnuella soli gen. nov., sp. nov., a bacterium from soil belonging to the family Chitinophagaceae

A Gram-stain negative, aerobic, rod-shaped, yellow-coloured bacterium, designated strain 17J28-1(T), was isolated from soil of Jeju Island, Korea. Optimal growth was found to occur at 30 degrees C, at pH 6.0 and in the absence of NaCl on R2A at 30 degrees C. Strain 17J28-1(T) showed resistance to UV-radiation. The draft genome sequence of strain 17J28-1(T) is 4804,510 bp long with a 49.4% G + C content. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 17J28-1(T) forms a distinct lineage within the family Chitinophagaceae (order Chitinophagales, class Chitinophagia) and is closely related to Cnuella takakiae (93.7% 16S rRNA gene sequence similarity) and Flavisolibacter ginsengiterrae (93.1%). In addition, genomic comparison using digital DNA-DNA hybridization (dDDH) and OrthoANI analyses between the novel organism and the members of the family Chitinophagaceae revealed identities of 65.5-74.1% and 18.1-28%, respectively. The predominant cellular fatty acids were identified as C-15:0 iso, C-15:1 iso G and C-17:0 iso 3-OH and the major respiratory quinone as MK-7. Based on these data we propose that strain 17J28-1(T) (= KCTC 62223(T) = JCM 33202(T)) represents a novel genus and species in the family Chitinophagaceae, for which the name Pseudocnuella soli gen. nov. sp. nov. is proposed.

Automated summary

The bacterium strain 17J28-1(T) isolated from soil in Jeju Island, Korea represents a novel genus and species in the family Chitinophagaceae, named Pseudocnuella soli gen. nov. sp. nov. It shows specific growth advantages and resistance under certain conditions.