Journal
ANTICANCER RESEARCH
Volume 40, Issue 12, Pages 6765-6768Publisher
INT INST ANTICANCER RESEARCH
DOI: 10.21873/anticanres.14699
Keywords
3-D culture; histoculture; Gelfoam; patient-derived cancer cells; PDCC; patient-derived xenograft; PDX
Categories
Funding
- Yokohama City University research grant KAMOME Project
- Robert M. Hoffman Foundation for Cancer Research
Ask authors/readers for more resources
Background/Aim: The direct placement of patient tumors in 2-D culture on plastic or glass surfaces has inhibited the establishment of patient-derived cancer cells (PDCCs). The aim of the present study was to develop universal and efficient methods to prepare PDCCs. Materials and Methods: Fragments of patient-derived xenograft (PDX) tumors established form colon cancer liver metastasis (1 mm(3)) were placed on Gelfoam and cultured in DMEM. Results: PDX tumor fragments were cultured on Gelfoam. Cancer cells migrated from the explant and formed distinct 3-D structures in the Gelfoam. Each of the three PDCCs showed a distinct morphology. The cultures were essentially all cancer cells without fibroblasts, the opposite of what usually occurs in 2-D culture on plastic or glass. Gelfoam cultures could be readily passaged from one Gelfoam cube to anothers suggesting indefinite culture potential. Conclusion: A potentially universal method to establish PDCC using PDX tumors and 3-D Gelfoam histoculture was developed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available