Responsive Emulsions for Sequential Multienzyme Cascades

Multienzyme cascade biocatalysis is an efficient synthetic process, avoiding the isolation/purification of intermediates and shifting the reaction equilibrium to the product side.. However, multienzyme systems are often limited by their incompatibility and cross-reactivity. Herein, we report a multi-responsive emulsion to proceed multienzyme reactions sequentially for high reactivity. The emulsion is achieved using a CO2, pH, and thermo-responsive block copolymer as a stabilizer, allowing the on-demand control of emulsion morphology and phase composition. Applying this system to a three-step cascade reaction enables the individual optimal condition for each enzyme, and a high overall conversion (ca. 97 % of the calculated limit) is thereby obtained. Moreover, the multi-responsiveness of the emulsion allows the facile and separate yielding/recycling of products, polymers and active enzymes. Besides, the system could be scaled up with a good yield.

Automated summary

The novel approach of multi-enzyme cascade biocatalysis utilizes a multi-responsive emulsion to achieve high reactivity, individual optimal conditions for each enzyme, and high overall conversion. Furthermore, the multi-responsiveness of the emulsion facilitates the easy and separate yielding/recycling of products, polymers, and active enzymes, allowing for scalability with good yield.