Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 60, Issue 10, Pages 5157-5161Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202013011
Keywords
diarylethene; potential-free nanopipette; precise dosing; single-cell operation; supramolecular hydrogel
Categories
Funding
- NSFC [21878086, 21788102, 22020102006]
- Shanghai Rising-Star Program [19QA1402500]
- Shanghai Municipal Science and Technology Major Project [2018SHZDZX03]
- international cooperation program of Shanghai Science and Technology Committee [17520750100]
- Fundamental Research Funds for the Central Universities [222201717003]
- Shanghai Sailing Program [20YF1410300]
- China Postdoctoral Science Foundation [2020M671017]
Ask authors/readers for more resources
The newly developed photo-responsive hydrogel-nanopipette hybrid system enables precise single-cell operation with negligible cell damage, overcoming traditional issues associated with nanopipette operations. The light-triggered injection allows for precisely dose-controllable single-cell drug delivery, significantly reducing lethal doses of doxorubicin.
Single-cell and in situ cell-based operation with nanopipette approach offers a possibility to elucidate the intracellular processes and may aid the improvement of therapy efficiency and precision. We present here a photo-responsive hydrogel-nanopipette hybrid system that can achieve single-cell operation with high spatial/temporal resolution and negligible cell damage. This strategy overcomes long-time obstacles in nanopipette single-cell studies as high electric potential (ca. 1000 mV) or organic solvent is always used during operations, which would inevitably impose disturbance and damage to targeted cells. The light-triggered system promotes a potential-free, non-invasive single-cell injection, resulting in a well-retained cell viability (90 % survival rate). Moreover, the photo-driven injection enables a precisely dose-controllable single-cell drug delivery. Significantly reduced lethal doses of doxorubicin (163-217 fg cell(-1)) are demonstrated in corresponding cell lines.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available