Journal
ANALYTICAL SCIENCES
Volume 37, Issue 1, Pages 17-26Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.20SAR18
Keywords
DNA aptamer; SELEX; separation techniques
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Funding
- Japan Society for the Promotion of Science KAKENHI [JP19H02740]
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This review discusses the advancements in separation techniques for SELEX-based aptamer selection developed in this decade, including CE, microfluidic chip, solid phase, and FACS-based SELEX among others.
DNA aptamers, which are short, single-stranded DNA sequences that selectively bind to target substances (proteins, cells, small molecules, metal ions), can be acquired by means of the systematic evolution of ligands by exponential enrichment (SELEX) methodology. In the SELEX procedure, one of the keys for the effective acquisition of high-affinity and functional aptamer sequences is the separation stage to isolate target-bound DNA from unbound DNA in a randomized DNA library. In this review, various remarkable advancements in separation techniques for SELEX-based aptamer selection developed in this decade, are described and discussed, including CE-, microfluidic chip-, solid phase-, and FACS-based SELEX along with other methods.
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