Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 4, Pages 2480-2489Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c04518
Keywords
-
Categories
Funding
- National Nature Science Foundation of China [21675093, 21505081, 21974075]
- Natural Science Foundation of Shandong Province of China [ZR2019YQ13]
- Science and Technology Support Plan for Youth Innovation of Colleges and Universities in Shandong Province [2019KJC007]
- Research Foundation for Youth Scholars of Beijing Technology and Business University [19002020177]
- Beijing Technology and Business University Research Team Construction Project [PXM2020_014213_000017]
- Taishan Scholar Program of Shandong Province, China [ts20110829]
Ask authors/readers for more resources
A simple and powerful Au NST@SiO2-based PEF probe with 20 symmetric hot spots was developed for highly sensitive intracellular miRNA imaging. By regulating the thickness of the silica shell, the distance between Au NSTs and fluorescent dyes was controlled, resulting in an optimal fluorescence enhancement (21-fold).
Plasmon-enhanced fluorescence (PEF) is considered to be a powerful signal amplification technology to overcome intrinsic shortcomings of photobleaching and brightness of the traditional fluorescent dyes. Nevertheless, exploitation of PEF-based probes for bioimaging application is still at a very early stage. In this work, a simple but powerful gold nanostar (Au NST)@SiO2-based PEF probe with 20 symmetric hot spots was developed for highly sensitive lighting up in situ imaging of intracellular microRNAs (miRNAs). By regulating the thickness of the silica shell, the distance between Au NSTs and fluorescent dyes was controlled, and the optimum fluorescence enhancement (21-fold) was obtained with the silica shell thickness of approximately 22 nm. Thanks to the 20 more powerful hot spots that can produce stronger localized electric fields, the Au NST-based PEF probe exhibits stronger PEF effects than the traditional plasmonic nanostructures such as gold nanorods (Au NRs), gold nanobipyramids (Au NBPs), and triangular gold nanoprisms (Au NPRs), resulting in high sensitivity and improved detection limit (LOD) of 0.21 pM for miRNA-21 analysis. Moreover, not only cancer cells (MCF-7 and Hela) and normal cells (L02) with distinct miRNA-21 expression levels can be discriminated but also tumor cells in cocultured mixtures can be recognized, indicating its promising potential in clinical diagnosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available