Journal
ANALYTICAL CHEMISTRY
Volume 93, Issue 4, Pages 2090-2096Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.0c03836
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Funding
- National Key RD Program [2016YFD0500706]
- National Natural Science Foundation of China [21778020, 21804046]
- Sci-tech Innovation Foundation of Huazhong Agriculture University [2662017PY042]
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A novel PCN-224-based electrochemiluminescence (ECL) system was constructed for sensitive detection of PEDV with the use of TiO2 nanoparticles to amplify the ECL signal. This system achieved a sensitive detection range of 1 pg/mL to 10 ng/mL for PEDV.
The sensitive detection of coronavirus is of vital importance for the prevention of its rapid spread. Porcine epidemic diarrhea virus (PEDV) is a highly contagious coronavirus that causes severe diarrhea and death in neonatal piglets. In this work, a novel PCN-224-based electrochemiluminescence (ECL) system was constructed for PEDV detection with high sensitivity. We found that PCN-224 can be employed as an ECL reporter with a strong signal because of its zirconium-based organic porous frame nanomaterial with a large specific surface area and stable structure. TiO2 nanoparticles were used as an accelerator for the first time to promote the reduction of coreactant potassium peroxydisulfate on the cathode; thus, the initial ECL signal of PCN-224 was significantly amplified. In the presence of PEDV, the ECL signal decreased due to the block effect to electron transfer. As a result, the novel signal off biosensor achieved a sensitive detection of PEDV ranging from 1 pg/mL to 10 ng/mL, with a detection limit of 0.4 pg/mL (S/N = 3). Importantly, the PCN-224 nanomaterial enriched the ECL system in biological analysis, and the proposed strategy provided a new route for coronavirus detection.
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