Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 413, Issue 4, Pages 1027-1037Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00216-020-03061-1
Keywords
PbS quantum dots; Functionalization; Electrochemical DNA sensor; HEV3
Funding
- Thailand Research Fund [RSA6080050]
- KMUTT 55th Anniversary Commemorative Fund
- Petchra Pra Jom Klao Doctoral, KMUTT
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This study aimed to develop a highly specific electrochemical DNA sensor using functionalized lead sulphide quantum dots for detecting hepatitis E virus genotype 3 DNA target, showing low detection and quantitation limits.
The aim of this study was to develop a highly specific electrochemical DNA sensor using functionalized lead sulphide (PbS) quantum dots for hepatitis E virus genotype 3 (HEV3) DNA target detection. Functionalized-PbS quantum dots (QDs) were used as an electrochemical label for the detection of HEV3-DNA target by the technique of square wave anodic stripping voltammetry (SWASV). The functionalized-PbS quantum dots were characterized by UV-vis, FTIR, XRD, TEM and zeta potential techniques. As-prepared, functionalized-PbS quantum dots have an average size of 4.15 +/- 1.35 nm. The detection platform exhibited LOD and LOQ values of 1.23 fM and 2.11 fM, respectively. HEV3-DNA target spiked serum is also reported.
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