Journal
ACTA TROPICA
Volume 213, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.actatropica.2020.105761
Keywords
Extracellular vesicles; Trichinella spiralis; DSS-induced colitis; Immunomodulation; Macrophage
Categories
Funding
- National Key Research and Development Program of China [2017YFC1601206, 2017YFD0501302]
- National Natural Science Foundation of China [NSFC 31520103916, 31872467]
- Program for JLU Science and Technology Innovative Research Team
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The study demonstrates that Trichinella spiralis EVs can influence the development of inflammation in DSS-induced colitis by inhibiting M1 macrophage polarization, showcasing their immunomodulatory ability.
Extracellular vesicles (EVs) are membranous containers released by cells that are powerful agents of intercellular communication. EVs have been described for various parasites and are associated with tissue inflammation. Several studies have demonstrated that parasite EVs can have either proor anti-inflammatory impacts, depending on the type of parasite. To evaluate the immunomodulatory properties of EVs produced by Trichinella spiralis (T. spiralis), we established a mouse model with dextran sulphate sodium (DSS)-induced colitis. The muscle larvae of T. spiralis were cultured in vitro and the released EVs were isolated by ultracentrifugation. T. spiralis EVs (Ts-EVs) were characterized according to morphology, size and constituent surface proteins (CD63, Enolase and Hsp70). Mice were treated with water containing 3% DSS after last intraperitoneal injection of TsEVs. Disease activity index (DAI), macroscopic and histopathological scores of Ts-EVs group was lower than DSS group. And Ts-EVs prevented the increase in the expression of TNF-alpha, IFN-gamma, IL-17A and IL-1 beta observed in the colon of DSS-treated mice. In contrast, upregulation of IL-4, IL-10, TGF-beta and IL-13 expression was detected in TsEVs+DSS group. In addition, Ts-EVs increased the infiltration of alternatively activated (M2) macrophages into the colon. The expression of CD206 (M2 marker) in the mesenteric lymph nodes (MLN) of mice with colitis increased in Ts-EVs+DSS group. Furthermore, Ts-EVs interfered with both the NF-kappa B and MAPK signalling pathways. Taken together, our findings demonstrate that Ts-EVs can affect the development of inflammation in DSS-induced colitis by inhibiting M1 macrophage polarization, due to their immunomodulatory ability.
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