Journal
ACTA BIOMATERIALIA
Volume 119, Issue -, Pages 211-224Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2020.11.013
Keywords
covalent grafting; compliance match; anti-acute thrombosis; rapid endothelialization; vascular regeneration
Funding
- National Natural Science Foundation of China [81902186, 31900949]
- Shanghai Rising-Star Program [20QC1401300]
- China Postdoctoral Science Foundation [2019M661525]
- Shanghai Post-doctoral Excellence Program
- Science and Technology Commission of Shanghai Municipality [19440741300, 19441901700, 19441901701, 19441901702]
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The study synthesized PEUU@PEG-Hep vascular graft with good biocompatibility and blood compatibility, and the covalent grafting of PEG and heparin significantly enhanced endothelial cell adhesion, spreading, and proliferation, as well as improved the grafts' ability to prevent platelet deposition.
Rapid endothelialization of small-diameter vascular grafts remains a significant challenge in clinical practice. In addition, compliance mismatch causes intimal hyperplasia and finally leads to graft failure. To achieve compliance match and rapid endothelialization, we synthesized low-initial-modulus poly(esterurethane)urea (PEUU) elastomer and prepared it into electrospun tubular grafts and then functionalized the grafts with poly(ethylene glycol) (PEG) and heparin via covalent grafting. The PEGand heparinfunctionalized PEUU (PEUU@PEG-Hep) graft had comparable mechanical properties with the native blood vessel. In vitro data demonstrated that the grafts are of good cytocompatibility and blood compatibility. Covalent grafting of PEG and heparin significantly promoted the adhesion, spreading, and proliferation of human umbilical vein endothelial cells (HUVECs) and upregulated the expression of vascular endothelial cell-related genes, as well as increased the capability of grafts in preventing platelet deposition. In vivo assessments indicated good biocompatibility of the PEUU@PEG-Hep graft as it did not induce severe immune responses. Replacement of resected carotid artery with the PEUU@PEG-Hep graft in a rabbit model showed that the graft was capable of rapid endothelialization, initiated vascular remodeling, and maintained patency. This study demonstrates the PEUU@PEG-Hep vascular graft with compliance match and efficacious antithrombosis might find opportunities for bioactive blood vessel substitutes. (C) 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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