Bacterial Biofilms on Polyamide Nanofibers: Factors Influencing Biofilm Formation and Evaluation

Electrospun polyamide (PA) nanofibers have great potential for medical applications (in dermatology as antimicrobial compound carriers or surgical sutures). However, little is known about microbial colonization on these materials. Suitable methods need to be chosen and optimized for the analysis of biofilms formed on nanofibers and the influence of their morphology on biofilm formation. We analyzed 11 PA nanomaterials, both nonfunctionalized and functionalized with AgNO3, and tested the formation of a biofilm by clinically relevant bacteria (Escherichia coli CCM 4517, Staphylococcus aureus CCM 3953, and Staphylococcus epidermidis CCM 4418). By four different methods, it was confirmed that all of these bacteria attached to the PAs and formed biofilms; however, it was found that the selected method can influence the outcomes. For studying biofilms formed by the selected bacteria, scanning electron microscopy, resazurin staining, and colony-forming unit enumeration provided appropriate and comparable results. The values obtained by crystal violet (CV) staining were misleading due to the binding of the CV dye to the PA structure. In addition, the effect of nanofiber morphology parameters (fiber diameter and air permeability) and AgNO3 functionalization significantly influenced biofilm maturation. Furthermore, the correlations between air permeability and surface density and fiber diameter were revealed. Based on the statistical analysis, fiber diameter was confirmed as a crucial factor influencing biofilm formation (p <= 0.01). The functionalization of PAs with AgNO3 (from 0.1 wt %) effectively suppressed biofilm formation. The PA functionalized with a concentration of 0.1 wt % AgNO3 influenced the biofilm equally as nonfunctionalized PA 8% 2 g/m(2). Therefore, biofilm formation could be affected by the above-mentioned morphology parameters, and ultimately, the risk of infections from contaminated medical devices could be reduced.

Automated summary

The study examined the formation of biofilms on electrospun polyamide nanofibers, finding that various bacteria could attach and form biofilms on these materials, with different methods yielding varying outcomes. Factors such as fiber diameter, air permeability, and AgNO3 functionalization were shown to significantly influence biofilm formation. Additionally, statistical analysis confirmed that fiber diameter was a crucial factor in biofilm formation, and functionalization with AgNO3 effectively suppressed biofilm formation, potentially reducing the risk of infections from contaminated medical devices.