Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 12, Issue 51, Pages 56723-56730Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c16208
Keywords
synthetic extracellular matrices (ECM); stem cell secretome; polyisocyanide hydrogels; interleukin-10 (IL-10); wound healing
Funding
- European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie [642687]
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The application of stem cell-derived secretome in regenerative therapies offers the key advantage that instead of the stem cells, only their effective paracrine compounds are in vivo delivered. Ideally, the secretome can be steered by the culture conditions of the stem cells. So far, most studies use stem cells cultured on stiff plastic substrates, not representative of their native 3D environment. In this study, cells are cultured inside synthetic polyisocyanide (PIC)-based hydrogels, which are minimal, tailorable, and highly reproducible biomimetic matrices. Secretome analysis of human adipose-derived stem cells (multiplex, ELISA) displays that matrix manipulation is a powerful tool to direct the secretome composition. As an example, cells in nonadherent PIC gels secrete increased levels of IL-10 and the conditioned media from 3D culture accelerate wound closure. In all, our PIC-based approach opens the door to dedicated matrix design to engineer the secretome for custom applications.
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