Journal
CYTOTECHNOLOGY
Volume 68, Issue 6, Pages 2613-2623Publisher
SPRINGER
DOI: 10.1007/s10616-016-9985-y
Keywords
OPG; Periodontal tissue defects; Gene therapy; Tissue regeneration
Categories
Funding
- International Cooperation Research and Develop Project of Nanjing Health Bureau [201303051]
- Six Talent Peaks of High Level Talent Selection and Training Project of Jiangsu Province [2013-SWYY-006]
- Research Fund for the Doctoral Program of Higher Education of China [20093518110002]
- Science and Technology Bureau of Jiangsu Province [BL2013002]
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This study evaluated the ability of human osteoprotegerin gene-modified autologous periodontal ligament cells (PDLCs) in combination with cell transplantation to promote periodontal regeneration in beagle dogs. Adenovirus Ad5-hOPG-EGFP-transfected PDLCs and BME-10X collagen membranes were fabricated and used for periodontal repair. Buccal periodontal defects (mesiodistal width x depth: 5 x 5 mm) were created on the second, third, and fourth mandibular premolars in six normal beagle dogs, and the defects were histologically and histomorphometrically assessed for periodontal regeneration in the following four groups: (1) hOPG-PDLCs + BME-10X, (2) mock-PDLCs + BME-10X, (3) PDLCs + BME-10X, and (4) BME-10X. The radiographic and histological results suggested that hOPG-PDLCs significantly promoted periodontal defect repair. This study demonstrates the potential of hOPG-modified PDLCs for periodontal tissue regeneration.
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