Journal
BIOACTIVE MATERIALS
Volume 5, Issue 3, Pages 458-467Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.bioactmat.2020.03.009
Keywords
3D printing; Orthopaedic screws; Biodegradable; Porous screws
Funding
- Indo-US Science and Technology [Forum-JC-26-2014]
- National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health [R01EB020640]
- Connecticut Regenerative Medicine Research Fund [15-RMBUCHC-08]
- Council of Scientific and Industrial Research, Government of India [09/1095(0022)/18-EMR-I]
- Innovation in Science Pursuit for Inspired Research (INSPIRE), DST, India [IF150843]
Ask authors/readers for more resources
Advent of additive manufacturing in biomedical field has nurtured fabrication of complex, customizable and reproducible orthopaedic implants. Layer-by-layer deposition of biodegradable polymer employed in development of porous orthopaedic screws promises gradual dissolution and complete metabolic resorption thereby overcoming the limitations of conventional metallic screws. In the present study, screws with different pore sizes (916 x 918 mu m to 254 x 146 mu m) were 3D printed at 200 mu m layer height by varying printing parameters such as print speed, fill density and travel speed to augment the bone ingrowth. Micro-CT analysis and scanning electron micrographs of screws with 45% fill density confirmed porous interconnections (40.1%) and optimal pore size (259 x 207 x 200 mu m) without compromising the mechanical strength (24.58 +/- 1.36 MPa). Due to the open pore structure, the 3D printed screws showed increased weight gain due to the deposition of calcium when incubated in simulated body fluid. Osteoblast-like cells attached on screw and infiltrated into the pores over 14 days of in vitro culture. Further, the screws also supported greater human mesenchymal stem cell adhesion, proliferation and mineralized matrix synthesis over a period of 21 days in vitro culture as compared to non-porous screws. These porous screws showed significantly increased vascularization in a rat subcutaneous implantation as compared to control screws. Porous screws produced by additive manufacturing may promote better osteointegration due to enhanced mineralization and vascularization.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available