Journal
CYTOMETRY PART A
Volume 89A, Issue 9, Pages 799-802Publisher
WILEY
DOI: 10.1002/cyto.a.22932
Keywords
NK cell functions; KIR3DL05; KIR3DL01; rhesus macaque
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Funding
- NIH [5P51OD011106-54, R01 AI095098]
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This panel was developed to measure the functional capability of natural killer (NK) cell subsets in rhesus macaques (Macaca mulatta). It includes markers to determine the frequency of cytokine secreting and cytotoxic NK cell subpopulations in peripheral blood mononuclear cell (PBMC) samples stimulated in vitro with human 721.221 cells. NK cell subsets were defined by the expression of killer cell immunoglobulin-like receptors (KIRs) Mamu-KIR3DL01 and Mamu-KIR3DL05, and differentiation antigens CD16 and CD56. The panel can be used to assess the functional capability of NK cells in a range of normal and pathologic conditions of captive bred rhesus macaques of Indian origin. (c) 2016 International Society for Advancement of Cytometry
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