13C CP/MAS NMR Can Discriminate Genetic Backgrounds of Rice Starch

Solid-state cross-polarization magic-angle spinning carbon-13 nuclear magnetic resonance (C-13 CP/MAS NMR) spectroscopy is used to analyze starch derived from plants including wheat, maize, and potato, but few reports have described its application to rice starch. Here, we combined C-13 CP/MAS NMR with deconvolution and subtraction methods to analyze rice lines including mutants that are deficient in at least one enzyme involved in amylose and/or amylopectin biosynthesis. We found that differences in the content of ordered structures between rice lines could be evaluated using C1 signal deconvolution and subtraction. The content of the V-type ordered structure increased with increasing amylose content. Furthermore, starch derived from a starch synthase (SS) IIIa/starch branching enzyme (BE) IIb-deficient mutant formed B- and V-type ordered structures and significantly more nonordered structures than the other rice lines. These data indicate that C-13 CP/MAS NMR analysis is useful for discriminating the genetic backgrounds of starch derived from different rice cultivars.