4.7 Article

Improving the Efficiency of Adventitious Shoot Induction and Somatic Embryogenesis via Modification of WUSCHEL and LEAFY COTYLEDON 1

Journal

PLANTS-BASEL
Volume 9, Issue 11, Pages -

Publisher

MDPI
DOI: 10.3390/plants9111434

Keywords

somatic embryogenesis; adventitious shoot formation; transcription factor; stem cell

Categories

Funding

  1. JSPS KAKENHI [JP26840102, 20K05953]
  2. MEXT KAKENHI [17H05831, 19H04850]
  3. Naito Foundation
  4. Funding Program for Next Generation World-Leading Researchers Program [GS030]
  5. Grants-in-Aid for Scientific Research [17H05831, 20K05953, 19H04850] Funding Source: KAKEN

Ask authors/readers for more resources

The induction of adventitious organs, such as calli, shoots, and somatic embryos, in tissue culture is a useful technique for plant propagation and genetic modification. In recent years, several genes have been reported to be adventitious organ inducers and proposed to be useful for industrial applications. Even though the Arabidopsis (Arabidopsis thaliana) WUSCHEL (WUS) and LEAFY COTYLEDON 1 (LEC1) genes can induce adventitious organ formation in Arabidopsis without phytohormone treatment, further improvement is desired. Here, we show that modifying the transcriptional repression/activation activities of WUS and LEC1 improves the efficiency of adventitious organ formation in Arabidopsis. Because WUS functions as a transcriptional repressor during the induction of adventitious organs, we fused it to an artificial strong repression domain, SUPERMAN REPRESSION DOMAIN X (SRDX). Conversely, we fused the strong transcriptional activation domain VP16 from herpes simplex virus to LEC1. Upon overexpression of the corresponding transgenes, we succeeded in improving the efficiency of adventitious organ induction. Our results show that the modification of transcriptional repression/activation activity offers an effective method to improve the efficiency of adventitious organ formation in plants.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available